High-Performance Liquid Chromatography (HPLC) Analysis

Sample Preparation Requirements

  • Samples must be appropriately prepared and filtered prior to submission. Filtration through a suitable 0.22 µm or 0.45 µm membrane filter compatible with the sample matrix and mobile phase is recommended.
  • Samples should contain no or minimal color-producing impurities.
  • The sample pH must be within the recommended operational stability range of the selected HPLC column and compatible with the mobile phase and instrument specifications.
  • The sample solvent composition should be compatible with the initial mobile phase conditions.
  • The client must provide the required analytical method, including (where applicable):
    • Target analytes and expected concentration range
    • HPLC column specifications
    • Mobile phase composition and gradient/isocratic conditions
    • Flow rate
    • Detection wavelength(s) or detector parameters
    • Injection volume
    • Expected retention times and calibration information (if available)

Charges

  • The cost for a single HPLC data acquisition run (maximum runtime: 30 minutes; flow rate: 1.0 mL min⁻¹) is LKR 8,000 per sample.

Additional charges will apply for:

  • Repeat analysis due to unsuitable sample preparation or sample incompatibility
  • Additional injections/trials requested by the client
  • Re-analysis arising from sample-related issues
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