Learning outcomes:

At the end of the course unit student should be able to

• carryout the detection & differentiation of microorganisms using DNA based techniques,
• analyze the biologically important components in the laboratory,
• demonstrate some physiological activities of microorganisms,
• demonstrate the potential usage of some microbial metabolic activities/processes in industries.

Course content:
Laboratory exercises to illustrate the contents and special features emphasized in lecture course MIBI 21014. Preparation of bacterial Genomic DNA, Preparation of bacterial plasmid DNA, Digestion of DNA and analysis of the digestions by Gel electrophoresis: Restricted Fragment Length Polymorphism (RFLP) analysis, Bacterial transformation and cloning of DNA, Polymerase Chain Reaction (PCR) for the detection of important Bacterial species and some viruses. Determination of bacterial enzymes, chromatography techniques.

Method of teaching and learning:

practical, demonstration classes and computer assisted learning.

Assessment:

Continuous assessment and end of the course unit examination.

Recommended reading:

1. Anderson, P and Ganetzky, B. (1997). An electronic companion to genetics workbook. Cogito Learning Media, Inc., New York
2. Creighton, T.E. (1990). Protein Structure a Practical Approach. IRL Press, New York,
3. John G. Holt (1994) Bergy's Manual of Determinative Bacteriology. Williams & Wilkins, Baltimore
4. Sambrook, J., Frtsch, E.F and Maniatis, T. (1989). Molecular Cloning- A Laboratory Manual. Vol. 1, 2, and 3. Cold Spring Harbor.